You are testing the growth parameters of a novel bacterial species you have isolated. To determine i Show more You are testing the growth parameters of a novel bacterial species you have isolated.
You are testing the growth parameters of a novel bacterial species you have isolated. To determine i Show more You are testing the growth parameters of a novel bacterial species you have isolated.
To determine its optimal growth temperature you will want to determine specific growth rates at various temperatures. To do so you are using the spread plate count method to assess viable culture counts at particular times. 1. Your first task will be to enumerate the number of viable cells in your stock culture flask so you may use them for your experiment. To do so you make a serial dilution (10 fold for each step) of the stock culture and from each dilution tube in the series you plate 0.1 ml on a separate nutrient agar plate via the spread plate method. Here are your counts from the dilution series as colony forming units (cfu the name for individual colonies in a plate count): Tube Count (cfu) 1) 10-1 TNTC 2) 10-2 350 3) 10-3 48 4) 10-4 6 5) 10-5 1 Using this information calculate the viable concentration of cells in the stock flask. Remember to think about which plate counts are accurate. Show less
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