Viruses in one milliliter were inc Show more The growth of the HPV was monitored as follows: 10 9 cells and 10 6 viruses in one milliliter were incubated under appropriate conditions.

Viruses in one milliliter were inc Show more The growth of the HPV was monitored as follows: 10 9 cells and 10 6 viruses in one milliliter were incubated under appropriate conditions.
Following a first infectious cycle a direct count and a plaque assay were performed. In the case of the direct count the mixture was diluted by a factor of 1000X after which beads were added to obtain a final concentration of 1 X 10 4 beads/mL. A microscopic field of vision revealed 20 beads and 150 virions. In the case of the plaque assay 67 plaques were observed on a plate on which 0.1 mL of a 10 -5 dilution was assayed. After the last infectious cycle a 10 -8 dilution was made on the supernatant. If 0.1 mL of this dilution is applied to a monolayer of cells how many plaques would you expect? Show less

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