Students will be given the opportunity to design and carry out a series of experiments into the toxicity of laboratory compounds using mammalian cultured cells.

Students will be given the opportunity to design and carry out a series of experiments into the toxicity of laboratory compounds using mammalian cultured cells.
Students will work in their mini-groups of three with each group looking at the toxicity of different compounds, including alcohols (ethanol and methanol), a range of “over the counter” medicines (e.g. salicylic acid (Aspirin), paracetamol and Anti-histamine) and antibiotics. The available options will be discussed during the first workshop.
Over the three weeks of the projects students will have to consider the following.
Week 1 – Planning, designing and initial optimisation of experiment
Groups should decide which cell type they wish to look at and why, we have CHO cells (hamster) and LTK cells (mouse), they may wish to look at responses in more than one cell type and see if there are any differences. They should consider that they need a confluence of cells of around 50-60% to be able to assess the effects of their compounds.
In this first session they should also decide what compound they wish to look at and at what range of concentrations they wish to look at, students should look at a concentration that gives variable responses. They should also consider what will happen when they add there compound to their culture the following week, will there be a dilution to take into account. They should also consider the number of replicates, size of multi-well dish to use and controls required. You should also generate a list of lab equipment that you will require during week 2, you should discuss these requirements with staff.
During this session staff will show you how to make a cell suspension and you will be able to practice how to count cells using the haemocytometer, this will give you an insight into the number of cells available from a stock flask of cells and should help you with designing your experiment.
Week 2 – Analysis of cell seeding densities, preparation of experimental samples and optimisation of toxicity assays
Students should then plate out their cells for adding their compound, they should have considered in their planning the number of replicates required and the controls they will need. They should use this time in the lab to make up their stock solutions, this could be Molar concentrations or % they need to think about what the compound they have is and how they wish to use it. Once they have done this and correctly added their compounds they should arrange with staff who will fix their cells on the Friday of this week to initially assess the effects of the compounds on their cells.

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